rat anti acc1 antibody  (Proteintech)

Journal: Physiological Genomics

Article Title: Changes in the phosphoproteome of brown adipose tissue during hibernation in the ground squirrel, Ictidomys tridecemlineatus

doi: 10.1152/physiolgenomics.00038.2017

Figure Lengend Snippet: Increased phosphorylation of ACC Ser79 in hibernating vs. euthermic ground squirrels. BAT extracts prepared in nondenaturing buffer (10 µg of protein in each lane) were subjected to SDS-PAGE in 7.5% (wt/vol) acrylamide gels and immunoblotted with anti-rat phospho Ser79 ACC1 antibody (at a dilution of 1/1,000), which detects phospho Ser80 in squirrel ACC1. A separate gel was run at the same time with identical amounts of extract for immunoblotting with anti-total ACC antibody and anti-PP2A catalytic subunit antibody (each at a dilution of 1/1,000) as a loading control. Top: blots for 4 individuals (euthermic vs. hibernator). At left, the histogram shows band intensities obtained with anti-phospho ACC for euthermic (white bars) vs. hibernating (black bars) animals relative to band intensities obtained with anti-total ACC. At right, the histogram shows band intensities obtained with anti-total ACC for euthermic (white bars) vs. hibernating (black bars) animals relative to band intensities obtained with anti-PP2A catalytic subunit. The data are mean relative band intensities ± SE, n = 4. Statistically significant differences were assessed by an unpaired Student's 2-sided t-test, *Significant difference compared with the corresponding euthermic value, P < 0.05.

Article Snippet: Anti-rat phospho Ser79 ACC1 rabbit polyclonal antibody (#07-303) and anti-total human ACC1 rabbit monoclonal antibody (#04-322) were from Millipore.

Techniques: Phospho-proteomics, SDS Page, Western Blot, Control

Journal: Physiological Genomics

Article Title: Changes in the phosphoproteome of brown adipose tissue during hibernation in the ground squirrel, Ictidomys tridecemlineatus

doi: 10.1152/physiolgenomics.00038.2017

Figure Lengend Snippet: Increased phosphorylation of ACC Ser79 in hibernating vs. euthermic ground squirrels. BAT extracts prepared in nondenaturing buffer (10 µg of protein in each lane) were subjected to SDS-PAGE in 7.5% (wt/vol) acrylamide gels and immunoblotted with anti-rat phospho Ser79 ACC1 antibody (at a dilution of 1/1,000), which detects phospho Ser80 in squirrel ACC1. A separate gel was run at the same time with identical amounts of extract for immunoblotting with anti-total ACC antibody and anti-PP2A catalytic subunit antibody (each at a dilution of 1/1,000) as a loading control. Top: blots for 4 individuals (euthermic vs. hibernator). At left, the histogram shows band intensities obtained with anti-phospho ACC for euthermic (white bars) vs. hibernating (black bars) animals relative to band intensities obtained with anti-total ACC. At right, the histogram shows band intensities obtained with anti-total ACC for euthermic (white bars) vs. hibernating (black bars) animals relative to band intensities obtained with anti-PP2A catalytic subunit. The data are mean relative band intensities ± SE, n = 4. Statistically significant differences were assessed by an unpaired Student's 2-sided t-test, *Significant difference compared with the corresponding euthermic value, P < 0.05.

Article Snippet: Anti-rat phospho Ser79 ACC1 rabbit polyclonal antibody (#07-303) and anti-total human ACC1 rabbit monoclonal antibody (#04-322) were from Millipore.

Techniques: SDS Page, Western Blot